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-arrestin 2 recruitment HEK293T cells (3.2 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 g plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and -arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent

Heterogeneity in the analyses vs active comparators could be attributed to differences in efficacy between the two GLP-1 RAs comparators (dulaglutide and semaglutide) or to differences in background glucose-lowering therapy between the two trials with basal insulin

Proper dosing schedules become essential because higher doses lead to more frequent side effects such as gastrointestinal upset, and the side effects can be managed by using the minimum effective dose or slow titration (Figure 2)

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