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16 L of this pooled probe mixture was combined with 40 L PEG-4000 (50% w/v), 40 L T4 DNA ligase buffer, 72 L water, and 2 L T4 DNA Ligase (30 U/L, Thermo Fisher)

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Future large-scale randomized trials with extended follow-up and standardized respiratory endpoints are warranted to clarify durability and causal mechanisms

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For example, some studies reported an average weight loss of 5.4% to 8.1% of starting body weight with Contrave, versus 1.3% to 4.9% with placebo

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