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This involved adding a lipid-ethanol solution to a plasmid solution in 25 mM citrate buffer (pH 4.0), followed by buffer replacement with PBS through diafiltration using Amicon Ultra Centrifuga Filter (10 kDa MWCO, Millipore)

Thieu VT, Robinson S, KennedyMartin T, Boye KS, GarciaPerez LE

doi: 10.3945/ajcn.114.084038

4 Potential emerging biomarkers of early atherosclerotic initiation Building on these established biomarkers, emerging molecular regulators such as circulating microRNAs (miRs) have gained increasing attention as early indicators of atherosclerotic initiation

Diabetes Ther 12(1):143157