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Bacterial culture was pelleted at 2,500 rpm for 30 minutes and resuspended in lysis buffer (0.1 M NaCl, 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 1X protease inhibitor cocktail) and 0.35 mg/mL lysozyme added for 30 minutes on ice
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Two to three sessions of 20 to 40 minutes at conversational pace enhances fat oxidation without interfering with recovery from resistance training
