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The cells were then fixed with 4% PFA at room temperature for 0.5 h and stained with Hoechst 33342 at room temperature for 10 min

When NNMT is overactive, it consumes S-adenosylmethionine (SAM) and reduces levels of nicotinamide adenine dinucleotide (NAD+), a critical coenzyme involved in cellular energy production

The Ozempic FDA label specifically warns about the risk of dehydration from these stomach side effects, which is why staying well hydrated matters

doi: 10.1038/s41571-025-01085-z

Ire1 in POMC neurons is required for thermogenesis and glycemia

Mild Headaches: A less common but reported side effect
