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After washing, the membranes were incubated with secondary antibodies conjugated with horseradish peroxidase diluted at 1:5000 in TBST for 1 h at room temperature

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Hld Se was suspended in Krebs buffer containing 0.2% w/v BSA and 0.03% w/v bovine bile, at 20 and 40 M and incubated on the NGN3-HIE monolayers for 2 h

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