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Preparation of silica-induced macrophage-conditioned media The MH-S mouse alveolar macrophage cell line was purchased from Procell (Wuhan, China) and cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/ml penicillin, and 100 g/ml streptomycin at 37 C and 5% CO 2

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Glucagon Receptor: Increases energy expenditure, promotes fat breakdown, and directly supports the reduction of fat in the liver

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