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Amplified DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and plasmid DNA from newly constructed vectors was isolated using the NucleoSpin Plasmid EasyPure kit (Macherey-Nagel)

Brain tissues were collected, fixed in 4% paraformaldehyde for at least 24 hours, dehydrated in graded alcohol, cleared with xylene, and embedded in paraffin

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Second Nature pairs medication with support from registered dietitians and a structured programme built around the balanced plate and lasting habit change

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Improved Blood Sugar Control Even with lower doses, semaglutide has been shown to help regulate blood glucose by enhancing insulin sensitivity, slowing gastric emptying, and reducing glucagon secretion