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The rat Gcg promoter comprises at least 5 cis-acting elements (G 1 G 5 ) plus a cAMP response element (CRE), all of which are located within the 2.5 kb region upstream of the Gcg transcription start [89,94,[101], [102], [103]]

[Google Scholar] 4.Muller T.D., Finan B., Clemmensen C., DiMarchi R.D., Tschop M.H

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GAPDH was used as a loading control ( n = 3).( B ) cAMP levels in coronary effluents were measured at timed intervals during an initial 10-minute perfusion of WT hearts with either 6 nM GLP-1(2836), scrambled(2836) control (Scram), or buffer-only control (Cntl), or with 0.3 nM GLP-1, and were normalized to coronary flow (mL/min/g heart weight)