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Another study demonstrated that empagliflozin appears to primarily reduce oxidative stress in cardiomyocytes by inhibiting NHE1, consequently enhancing cardiomyocyte stiffness, promoting extracellular matrix remodelling, mitigating centripetal hypertrophy of the heart, and alleviating systemic inflammation [119]

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MIN6B1 cells were incubated overnight with serum-free media plus 25 mM glucose and 0.5 mM palmitate/BSA 100 nM agonists, fixed, processed with the In Situ Cell Death Detection Kit, TMR red (Roche), and imaged as above

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