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Cells were washed with standard extracellular buffer (Hepes-NaOH, pH7.5, 143.4 mM NaCl, 4.5 mM KCl, 2.6 mM CaCl 2 , 1.2 mM MgCl 2 , 5.5 mM glucose) containing 5 M Fura-8AM (AAT Bioquest, Sunnyvale, CA, USA) for 15 min at 37 C
However, due to danugliprons potential for drug-induced liver damage, 2025 Pfizer has announced that it is discontinuing development (Pfizer, 2025)
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Drug Treatment of Child And Adolescent Obesity
(2024) 261:e230342
Increased GIP signaling induces adipose inflammation via a HIF-1alpha-dependent pathway and impairs insulin sensitivity in mice
