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Peptide Purification The cells (from 100 mL culture) were harvested by centrifugation (4 C, 10,000 g , 5 min), resuspended in 20 mL of lysis buffer (20 mM NaH 2 PO4, 300 mM NaCl, 10 mM imidazole, pH 7.4), and lysed by sonication (10 s ON, 10 s OFF, 4555% amplitude, 1015 min)

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Introducing an angiogenic peptide to a vHL patient poses severe health risks

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