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Based on our prior experience, 22 NanoBiT strategy 23,24 was used in this study to stabilize the GLP-2GLP-2RG s complex and strengthen the interactions between GLP-2R and G, resulting in a cryo-EM structure at a global resolution of 3.0 (Supplementary information, Table S1)

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When we treated the slices with 100 nM tertiapin-Q, a GIRK channel blocker, membrane potential was depolarized by 2.90.3 mV (from 52.42.1 to 49.51.9 mV, n =4) in four of seven POMC neurons (50%) (Fig