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Briefly, sections were fixed in Bouin's solution at 56C for 1 h to enhance staining, followed by staining with Weigert's iron hematoxylin for nuclei at room temperature for 10 min, Biebrich scarlet-acid fuchsin for cytoplasm and muscle fibers at room temperature for 10 min, and aniline blue for collagen at room temperature for 5 min

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