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The sample were then vortexed and centrifuged (15,000 g , 5 min, 16 C) to sediment the precipitated protein
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The sample was then thawed on ice, 1.7 ml of Methyl tert-butyl ether (MTBE) was added, and the sample was vigorously vortexed
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T., Baildam A., and Howell A., Energy Balance in Early Breast Cancer Patients Receiving Adjuvant Chemotherapy, Breast Cancer Research and Treatment 83 (2004): 201210
