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( B ) Quantification of WIPI2 puncta in Fed and starved (2 hours with Earles balanced salt solution [EBSS]) conditions in WT and Mytho -KO C2C12 cells in N = 3

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Collectively, the combined effects of prolonged hyperglycemia, obesity-driven hormonal imbalance, and androgen deprivation promote the development of a chronic pro-inflammatory milieu that favors progressive endothelial dysfunction [70]

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These movements can happen as often as every 15 seconds