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Never freeze: Freezing destroys the peptide structure and renders it inactive
The peptides were eluted with a linear gradient of solvent B from 35% to 50% for 30 min
41:2669-2701 [PMC free article: PMC6245208] [PubMed: 30291106] 8
Semaglutide's structure is best read as two engineered answers to those two problems
