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Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects

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Immediate medical attention is warranted for symptoms suggesting serious complications, including severe chest pain, difficulty breathing that does not resolve with calming techniques, loss of consciousness, or confusion that may indicate severe hypoglycemia or other acute medical conditions requiring urgent evaluation

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Discard tirzepatide immediately if it was exposed to temperatures above 86F (30C) or if it was frozen at any point

This can lead to reduced calorie intake and, over time, significant weight loss