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The resulting supernatants were mixed with NuPAGE LDS Sample Buffer (Invitrogen, #NP0007) and 2-mercaptoethanol (10%

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Because of the high unit price and total expense of these medications, countries are now deliberating on whether and how to cover them as treatments for obesity [64]

Burlington, MA, USA) according to the manufacturers instructions

Annals of Internal Medicine 168(1):30-38
