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M., Mozaffarian, D., & Kris-Etherton, P

Autophagy is a cellular clean-up process that takes place when the body has limited access to nutrients, such as during fasting

It has become one of the most extensively studied metabolic peptides and serves as the reference compound for modern GLP-1 research

But the study also has important limitations that you should understand

2009 Jan;29(2):198206

Sample sections were blocked in 5 % goat serum with 0.3 % Triton X-100 for 1 h at RT and then incubated overnight at 4 C with the following primary antibodies: mouse anti-GLP-1R (1:50, Santa Cruz, CA, USA), mouse anti-GLP-1(1:50, Santa Cruz, CA, USA), rabbit anti-Iba1 (1:500, Wako Chemicals, Tokyo, Japan), rabbit anti-GFAP (1:200, Cell Signalling, Boston, MA, USA), rabbit anti-NeuN (1:100, Proteintech, China), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), and mouse anti-c-fos (1:100, Santa Cruz, CA, USA)