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Using a PCR thermocycler (Applied Biosystems PCR system 9700, ThermoFisher, CA, United States), the DNA was initially denatured at 95C for 2 min, followed by 25 cycles of 98C for 30 s, 66C for 15 s and 72C for 3.5 min
GLP-1R/GIPR/GCGR signaling in neuroprotection
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Bioactivities of the GLP-1 peptides The earliest studies of the bioactivities of GLP-1(1-37) were indecisive
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Semaglutide, the ingredient in Ozempic, has been shown to protect the kidneys and reduce heart issues in those with chronic kidney disease and diabetes
