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The brain samples were embedded in the 4%UltraPure low melting point agarose (Invitrogen) in PBS on the ice

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Second, it is possible that some of the individuals categorized as nonsmokers in our data are former smokers who were able to quit because they were prescribed a GLP-1

Lifestyle factors also play a major role: patients who eat larger meals, consume acidic foods, lie down shortly after eating, or carry excess abdominal fat typically experience worse reflux on GLP-1

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