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Cells were cultured at 37 C in Dulbeccos modified Eagles medium (DMEM) + GlutaMax 1965 (Gibco) supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin in a humidified 10% CO 2 incubator

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Large self-funded employers have increasingly been adding GLP-1 coverage as employee demand grows, but many smaller employers and fully insured groups have not, primarily because the cost impact on premiums is significant