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Diabetologia 56, 14131416

2023;22(4): doi:10.36849/JDD.6550 Citation: Ouellette S, Frias G, Shah R, et al

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For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio

Both tirzepatide and semaglutide treatment led to similar improvements in fasted and postprandial appetite-related sensations (Table S1) and reduction of food intake at an ad libitum meal (348.4 kcal and 284.1 kcal, respectively

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