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allow it to reach room temperature before injection to reduce discomfort
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The slides were then blocked with 2% goat serum in 1 TBST for 1 h at room temperature, and incubated with anti-mouse PLIN2 antibody (Thermo Fisher Scientific Cat #15294-1AP), anti-mouse 4HNE antibody (Thermo Fisher Scientific Cat #MA5-27570) and anti-rabbit CYP2E1 antibody (Abcam Cat #ab28146)
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