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On the day of the experiment, cells were washed twice with 500 l nutrient-free KRB containing (mmol/l): 120 NaCl, 5 KCl, 2 CaCl 2 , 1 MgCl 2 , 22 NaHCO 3 , and 0.1 Diprotin A, bubbled with 95% O 2 /5% CO 2 for 10 min and supplemented with 0.5% (w/v) BSA
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