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Cells were imaged by time-lapse microscopy using a Zeiss LSM-780 inverted confocal laser-scanning microscope with a 63/1.4 NA oil immersion objective and a 488 nm excitation wavelength and emissions at 508 and 583 nm for 2 minutes to record a baseline, followed by agonist stimulation at 100 nM for 5 minutes and 2-minute exposure to 10 M FSK + 100 M IBMX to generate a maximal response

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This mode of regulation may enable interpretation and communication of extra-germline environmental cues, and thus optimize germ cell proliferation and progeny generation in ephemeral habitats and in pathophysiological conditions

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