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Competition binding with human GLP-1(7-36)NH 2 , GIP(1-42), tirzepatide, and semaglutide was performed essentially as described for homologous competition except that the assay buffer was 1.0 mM MgCl 2 , 2.5 mM CaCl 2 , 0.003% w/v Tween-20, 0.1% w/v bacitracin in 25 mM HEPES, pH 7.4, final concentrations with one Complete EDTA free protease inhibitor tablet added per 50 mL of buffer

In a similar model, intrathecal injections of exenatide inhibited thermal hyperalgesia and mechanical allodynia by enhancing spinal microglial expression of -endorphins, IL-10 and interleukin 4 (IL-4) [34, 35]

( 10.1136/hrt.2004.036905) [DOI] [PMC free article] [PubMed] [Google Scholar] Chen S Okahara F Osaki N & Shimotoyodome A

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