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TR-FRET signal from each well was then recorded using a Flexstation 3 with the following settings: ex 340 nm, em 520 and 620 nm, auto-cutoff, delay 50 s, integration time 300 s
Extracting extra or fractional doses from pens defeats the devices design, risks contamination, and can derail titration

Finally, lysosomal and liposomal colocalization was imaged with a high-content screening system in confocal mode, and Pearson correlation coefficients were calculated by using GraphPad Prism 8.3.0 software 67

doi: 10.1038/npp.2011.70
Concentration first
