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5 The research group intends to extend their data collection across additional imaging centres to provide a stronger evidence base for future national guidance

If the OD value at 620nm is lower than 0.8, the staining duration can be properly extended

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We used Primer-BLAST ( to design specific primers that met the following criteria: (1) Primer pairs are unique

10.1016/j.yexcr.2025.114769