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Once the optical density (OD 600 ) reached approximately 0.6, the culture was induced with 1 mM IPTG (Isopropyl -D-1-thiogalactopyranoside) and incubated for an additional 23 h at 37 C in a shaker

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On the other side, sustained GLP1R/cAMP signaling could dampen or alter the normal Ca 2+ oscillation pattern via Ca 2+ -cAMP cross-talk [72]

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