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Efficacy and safety of low and very low carbohydrate diets for type 2 diabetes remission: systematic review and meta-analysis of published and unpublished randomized trial data

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After washing the cultures three times with serum-free DMEM, GLP-1 release assays were performed for 30 minutes during which the cells were exposed to test compounds dissolved in serum-free DMEM containing 5.6 mM glucose and 0.1% BSA