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Cells were seeded in 96-well plates at a density of 35,000 cells per well and transiently transfected using Lipofectamine 2000 according to the manufacturers protocol

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Next, we estimated and compared the half-maximal inhibitory concentration (IC 50 ) values from the concentrationresponse data using Chinese hamster ovary (CHO K1) cells expressing human TRPV1

Some discover that rotating between all three gives them the best long-term experience

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