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Slices were transferred to carbogenated artificial cerebrospinal fluid (aCSF) solution (in mM: 127 NaCl, 25 NaHCO3, 1.25 NaH2PO4, 2.5 KCl, 25 D-glucose, 2 CaCl2, 1 MgCl2) at 37C for 30 minutes and then at room temperature for at least 45 minutes before recording

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By recognizing a limitless number of physio-pathological signals, macrophages rapidly acquire distinct immune phenotypes defined within two activation states, the classical, pro-inflammatory M1 and the alternative, anti-inflammatory M2 phenotypes

Grb7, a crucial protein associated with cancer cell proliferation and migration, is a noteworthy target of interest, particularly in breast cancer subtypes