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But drug manufacturers do not have much incentive to make such a deal because demand is already enormous for GLP-1s, Klebanoff said

Their finding marked a decisive breakthrough because it challenged a long-standing belief that nerves controlled the secretion of digestive enzymes that had grown out of the work of Ivan Pavlov, the Russian physiologist who demonstrated that dogs can be conditioned to salivate by the sound of a bell (Rehfeld 2012)

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However, the methods required to confirm specific receptors are limited, as antibodies need to be able to distinguish between cleaved and non-cleaved peptides, which differ by only two amino acids at the N-terminal end

Pretty soon he was growing colonies of bacteria spliced with bits and pieces of fish DNA and screening them with radioactive probes to identify the one carrying the gene for somatostatin