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p = 0.0001), 12 months: 0.47 (95% CI: 0.38, 0.58

4 and 5) were processed and analysed using bulk RNA-sequencing

The expression of TNF- , MCP-1, and F4/80 mRNA levels is increased in the testis and significantly decreased the sperm motility and activity in diet-induced obesity mice, and exenatide (24 nmol/kg/day) treatment suppressed the expression of TNF- , MCP-1, and F4/80 mRNA levels in testis and improved sperm quality in diet-induced obesity mice [111]

E.JansonJ.Bonner-WeirS.RitzelR.RizzaR

For co-staining with antibodies, EdU-labeled gonads were incubated in the primary antibodies after washing for three times, and subsequently in the secondary antibodies as described above