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6 Importance of the glycocalyx for the diagnosis and treatment of S-AKI 6.1 Challenges in the early diagnosis of S-AKI and potential biomarkers Currently, the fundamental problem regarding the treatment or prevention of S-AKI is that it is typically diagnosed late

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J Biol Chem (1996) 271:562832

Eluate incubated with TEV protease was dialysed against 25 mM Tris (pH 7.5), 150 mM NaCl and 1 mM DTT overnight at 4 C, then incubated with Ni-NTA resin for 1 h to remove the His-tagged protease

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