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NR supplement can increase the NAD + concentration, dependent on NRKs (NR kinases), thereby avoiding the need for NAMPT in the salvage synthesis pathway

The use of affinity tags and resins influence the purity, number of purification steps, protein yield, elution efficiency, and the cost

We also explored the effects of 2DG on key signaling pathways, such as AMP-activated protein kinase (AMPK), that regulate metabolic and inflammatory responses

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