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Increased NEFAs in cells compete with glucose for substrate oxidation, thus resulting in the inhibition of pyruvate dehydrogenase, phosphofructokinase, and hexokinase II [8]

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As was noted for DNMT1 and PRMT3, sigmoidal dose-response curves and reliable estimates of IC 50 values could not be obtained since no significant inhibition was observed at the tested NNMT inhibitor concentrations

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It reduces methylationdriven consumption of NAM, increases substrate availability for NAD+ synthesis, and promotes NAD+ biosynthesis to elevate intracellular NAD+ levels