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Water and food was available ad libitum, and whole rats were maintained in an environment in accordance with committee guidelines
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doi: 10.1016/j.cell.2013.12.010 45 DixonSJLembergKMLamprechtMRSkoutaRZaitsevEMGleasonCEet al
288 ZirnB.HartmannO.SamansB.KrauseM.WittmannS.MertensF.et al
10.1016/j.lwt.2015.11.004 64 LaytonA
The tissues were homogenized using a high-speed drill on ice in a glass Dounce homogenizer in Mitochondria Isolation Buffer (containing in mM 210 sucrose, 2 EGTA, 40 NaCl, 30 HEPES, pH 7.4)
