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Take supernatant, centrifugate at 8000~10000rpm (by low temperature high speed centrifuge) for 15 minutes, sediment is mitochondria

Patients carrying the GLP1R loss-of-function variant, for example, may not respond adequately to tirzepatide alone and could benefit from adjunctive growth hormone stimulation, whereas those with high receptor sensitivity may experience intolerable nausea from combination dosing