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Briefly, LOG cell cultures of yeast were diluted with PBS buffer to obtain suspensions with densities of 15 10 7 , 15 10 6 , 15 10 5 , 15 10 4 and 15 10 3 cells/mL, which were plated on solid YPD or YPGly medium containing 2% agar with various concentrations of DMSO using a stainless steel replicator

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