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doi: 10.3389/fpls.2019.00340 31 Garca-GarcaA
Tissues were homogenized in HEPES buffer, protein concentrations adjusted to 5.2 mg/ml and diluted 1:50 in Assay buffer
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Organoids were exposed to the drugs or the vehicle control (DMSO) through complete daily media changes and drug or vehicle replacement starting at DIV 7 and collected at DIV 20 or DIV 40, the two endpoints for analysis (Figure 1B)
De Felice FG, Gonalves RA, Ferreira ST
Wu Y, et al
