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Cancer 22 (1), 106

After 24 and 48 h of treatment, the treated and untreated nymphs or adult females (2 g) were homogenized in 20 ml of 0.1 M ice-cold phosphate buffer (pH 7.4) using a Polytron Kinemetica

Sadly, doctors will often stop treatment when B12 levels look high

Peptides were eluted with an organic solvent gradient from 100% phase A (0.1% FA) to 34% phase B (95% ACN, 0.1% FA) at a constant flow-rate of 250 nL/min

Epigenetic control of region-specific transcriptional programs in mouse cerebellar and cortical astrocytes

Afterward, they were incubated with primary antibody (listed in Additional file 2: Table S2) at room temperature for 2 h, washed with PBS, and incubated with Cy3- or FITC-labeled secondary antibody (listed in Additional file 3: Table S3) for 1 h