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Copper is an essential cofactor for a range of enzymes involved in follicle function, including lysyl oxidase (involved in extracellular matrix cross-linking) and superoxide dismutase (an antioxidant enzyme expressed within the follicle)

But the solution only lasts 28 days once mixed

Adenylate cyclases (of which 9 membrane-bound and one cytosolic forms are known in humans) may also be activated or inhibited in other ways (e.g., Ca2+/calmodulin binding), which can modify the activity of these enzymes in an additive or synergistic fashion along with the G proteins

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The recirculation of ICA-1s at the 12 h mark may have been variability of the experiment itself due to a requirement of an individual sacrifice per time point, but could also be an item to revisit