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Acknowledgment Figures created with BioRender.com ( Funding We appreciate the funding support from the NIH (R15 HL143545/HL/NHLBI)

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Lysates were thawed on ice, sonicated twice for 20 s, centrifuged at 14,000 g for 10 min at 4 C, and protein concentration was equalized

Since 2020, 8 of the 20 published clinical trials have focused on neurodevelopmental and neuropsychiatric/cognitive disorders, exhibiting a significant rise in clinical trials exploring sulforaphanes efficacy in the CNS compared to the previous two decades where only two of 57 trials focused on ASD alone with no published trials examining other CNS disorders (Supplementary Table 1)

Your physician determines which administration method best aligns with your goals