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The injection is followed by a few days of mild swelling, but it has a definitive effect on destroying stubborn fat cells

To induce DSBs, cells were treated with Z-(4)-Hydroxytamoxifen (Millipore Sigma, Cat#H7904, 1M) and Shield-1 (TaKaRa, Cat#632189, 1M) for a period of 4 hours in charcoal stripped FBS containing medium

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