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Description
For lentiviral induction (Day-0), 1 g/mL doxycycline (Sigma-Aldrich, Australia) was added to neural induction media [Neurobasal medium (Thermo, Australia), 1 N-2 supplement (Thermo, Australia), 1 B-27 with vitamin A supplement (Thermo, Australia), 1 Insulin-transferrin-Selenium-A (Thermo, Australia), 1 GlutaMAX TM (Life Technologies, USA)] supplemented with 10 M SB431524 (Stemcell Technologies, Canada) and 0.1 M LDN193189 (Stemcell Technologies, Canada)

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Determination of the effect of zinc on differentiative activity of PEDF in Y79 cells Neuronal differentiation of Y79 cells was performed by growing cells on poly-L-lysine/collagen-coated culture dishes in the presence of PEDF/zinc in conditioned medium obtained from differentiated SH-SY5Y cells (see above) 133

Ghaseminejad-Raeini 2 , M
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Zink beschermt de cellen tegen oxidatieve schade, speelt een rol in het celdelingsproces, draagt bij tot een normale DNA-synthese en speelt een rol bij een normale eiwitsynthese
