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[6] In specific relation to the immune system, glutathione deficiency is known to impair macrophage and T-cell function

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Cells were permeabilized with Cytofix/Cytoperm (BD Bioscience) for 20 min at 4 C in the dark and incubated with anti-IFN- (PerCP-Cy5.5

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After 72 h, the cells were harvested and lysed with 2.0 mL of 1.0% Triton X-100 in TBS (~10 10 6 cells/mL)

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