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Under this perspective, as reported in a systematic analysis for the Global Burden of Disease Study 2021 [1, 2], the age-standardized rates of deaths per 100,000 individuals attributed to 37 unique conditions affecting the nervous system that now include neurodevelopmental disorders, late-life neurodegeneration, and emergent conditions such as cognitive impairment following coronavirus disease 2019 (COVID-19), has decreased by 33.6% from 1990 to 2021

10.1016/s0006-3495(99)77318-2 276 HuguetF.MeletA.Alves de SousaR.LieutaudA.ChevalierJ.MaigreL.et al (2012)

marginale was used ( The first reaction mixture comprised 1.2 l dNTPs (2.5 mM), 0.15 l Taq polymerase (0.05 U), 1.5 l reaction buffer IB (1), 0.6 l of a solution containing the mixed primers (10 mM), and 10.05 l sterile ultra-pure water

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